IIT Indore, Khandwa Road, Simrol- 453552, Indore (MP)
Tel. +91-731-660- 3311
Email- sic@iiti.ac.in

Gas Chromatography – Mass Spectrometer (GC-MS)

A National Facility Indian Institute of Technology Indore

Created with Sketch.


GSMS-QP2010 Ultra


It is a versatile analytical tool for the separation and identification of non-polar / mid-polar compounds in the reaction mixture. Presently SIC is equipped with a GC-MS QP 2010 Ultra mass spectrometer from Shimadzu Analytical India Pvt. Ltd. The machine can be configured into 3 types of configurations viz. GC-MS, GC-TCD, GC-FID. GC-MS uses Electron Ionization (EI), Chemical Ionization (CI) since it has the capability to perform positive and negative ionization for molecular weight information of more complex samples. GC-MS uses helium as inert carrier gas. It is a very sensitive instrument and can detect up to PPM-level masses. The mass range is 10-1000 Da, which allows analysis over a wide range of low and high-molecular-weight compounds. NIST Mass spectra library is available for the separation/ identification of organic compounds and molecules. GC-TCD can be used for Gases samples analysis such as Hydrogen, Nitrogen, Carbon Monoxide, Carbon Dioxide, and Methane effectively. The GC-FID is well suited for the analysis of hydrocarbons such as methane, ethane, acetylene, etc., but also for organic substances containing hydrocarbons and for volatile organic compounds.

Sample requirement for GC-MS

  1. Sample Quantity: 20 mg
  2. Solubility: must be soluble in Methanol
  3. Molecular weight: 50 Dalton to 600 Dalton
  4. The sample must be volatile for GC-MS analysis


  • Identification and quantitation of volatile and semi-volatile organic compounds in complex mixtures
  • Determination of molecular weights and (sometimes) elemental compositions of unknown organic
  • compounds in complex mixtures
  • Structural determination of unknown organic compounds in complex mixtures by matching
  • Their spectra with reference spectra and by a priori spectral interpretation
  • Analysis Time
  • In addition to sample preparation time, the instrumental analysis time usually is fixed by the duration of the gas chromatographic run, typically between 20 and 100 min