Model
Single Molecule Imaging, Nikon
Features
Using Total internal reflection fluorescence microscopy (TIRFM) offers a unique environment to study the dynamics of individual molecules in an aqueous environment. It takes advantage of the evanescent field that is generated beyond a glass surface when laser light is totally reflected off the interface between two materials with different refractive indexes such as a fused silica slide and an aqueous buffer.
Applications
- Figure Showing the working principle of TIRFM Top panel shows the details of the optical components associated with the TIRFM set-up and the bottom panel highlights the total internal reflection of the laser light at the glass/water interface.
