IIT Indore, Khandwa Road, Simrol- 453552, Indore (MP)
Tel. +91-731-660- 3311
Email- sic@iiti.ac.in

Total Internal Reflection Fluorescence Microscopy(TIRFM)

A National Facility Indian Institute of Technology Indore

Created with Sketch.


Single Molecule Imaging, Nikon


Using Total internal reflection fluorescence microscopy (TIRFM) offers a unique environment to study the dynamics of individual molecules in an aqueous environment. It takes advantage of the evanescent field that is generated beyond a glass surface when laser light is totally reflected off the interface between two materials with different refractive indexes such as a fused silica slide and an aqueous buffer.


  • Figure Showing the working principle of TIRFM Top panel shows the details of the optical components associated with the TIRFM set-up and the bottom panel highlights the total internal reflection of the laser light at the glass/water interface.