HR-MS

Features

Bruker Daltonik, Benchtop easy-to-use, High-Performance Electrospray Ionization Quadrupole time-of-flight LC-MS spectrometer designed for exact mass and true isotopic measurements.
The Liquid Chromatography-Mass Spectrometry (LC-MS) is a powerful analytical technique with very high sensitivity and specificity. LC-MS is combination of Liquid Chromatography (LC) and Mass Spectrometry (MS). With the Liquid Chromatography (LC) the separation of components can be done and then the sample eluents from LC are transferred into Mass Spectrometry (MS) where the detection, identification and determination of masses of components can be done in presence of other components
LC-MS is a hyphenated technique used in combination with separation power of HPLC with detection power of Mass spectrometry. It is widely used in pharmaceutical, chemical, food, agrochemical industries, environmental and forensic applications. LC-MS is used for qualitative and quantitative determination of drug substances and biological samples. Also it is commonly used in drug research and quality control

Applications

Liquid chromatography-mass spectrometry (LC–MS) is a powerful analytical technique used for separation, identification, and quantification of both unknown and known compounds as well as to elucidate the structure and chemical properties of different molecules

In Pharmacokinetics: LC-MS is used in the study of absorption, metabolism, and excretion of drugs. Bio analytical methods are used for quantitative and structural elucidation of drugs and its metabolites in the biological samples (plasma, urine, saliva, serum etc.

In Bioavailability and Bioequivalence study: Comparative bioequivalence studies in which quantitative determination of drugs or metabolites is measured in biological matrix, pharmacodynamics, clinical trials and In-vitro dissolution tests.

In determination of molecular weights: LC-MS is used for determination of molecular weights of known and unknown compounds. It provides the information about molecular weight, structure, identification, quantity of sample components. LC-MS is used for determination of molecular masses of proteins, nucleic acids, polymers and peptides.

In determination of Assay of drug and intermediates: LC-MS is used in pharmaceutical industry for determination of assay of drug substances, drug products, intermediates and their related compounds.

In Agrochemical and pesticides industry: It is used in determination of different components present in the fertilizers and pesticides.

Environmental Applications: LC-MS is used for detection of phenyl urea herbicides, detection of low level of carbaryl in food.

Handled By Mr. Ghanashyam Bhavsar

ghanashyam@iiti.ac.in