LC-HRMS – SIC – A National Facility of IIT Indore

SIC

Sophisticated instrumentation center (SIC) was established in September 2011 to expedite the research program at IIT Indore. SIC has now emerged as one of the first such centers in the country providing extensive analytical instrument support to the users of the research fraternity across the country. A major advantage of SIC is its accessibility to the students within the institute as well as to other institutes and industries. A very healthy ratio of students to the time availability on instruments, and quick analysis of external samples makes it a very useful facility.

Make

Waters

Model

LC-HRMS instrument includes an ACQUITY H-Class Plus UPLC coupled with a Xevo G3 QTOF Mass Spectrometer

Description

The ACQUITY UPLC system consists of quaternary solvent manager (QSM), sample manager-flow through needle (SM-FTN), column manager, PDA detector and Refractive Index (RI) Detector. The Xevo G3 QTOF can be configured with ESI, APCI and ASAP probe to get nominal mass and accurate mass detection. The instrument utilises the power of MassLynx V 4.2 software for data acquisition and processing.

Features

Mass Range: TOF: 20 to 1,00,000 m/z

Quadrupole:
20-4000 m/z in resolving mode
20-16,000 m/z in non-resolving mode

Resolving power: > 40,000 FWHM
Mass accuracy: >1 ppm
Temperature range: SM-FTN: 4^O to 40^OC
Column manager: 25^O to 80 ^OC

Sample Requirements:

Powder samples: 10 mg

Liquid samples: 2 ml (0.5 mg/mL concentration)

Submission Form

Collect your Data

Applications

Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) is a powerful analytical technique used for separation, identification and quantification of both unknown and known compounds as well as to elucidate the structure of different molecules.
In determination of molecular weights: LC-HRMS is used for determination of molecular weights of known and unknown compounds. It provides information about molecular weight, structure, elemental composition and concentration of unknown sample in reaction mixture.
In Pharmaceutical Industries: It is used to determine the assay of drugs, intermediates, and related compounds.
In Pharmacokinetics: LC-MS is used to study of absorption, metabolism, and excretion of drugs. Bioanalytical methods are used for quantitative and structural elucidation of drugs and their metabolites in biological samples viz. plasma, urine, saliva and serum.
In Agrochemical and pesticides industry: It is used to determine different components present in fertilizers and pesticides.

Handled By Mr. Ghanashyam Bhavsar

ghanashyam@iiti.ac.in